Applications of Super Resolution Expansion Microscopy in Yeast

Super-resolution microscopy includes multiple techniques in optical that enable sub-diffraction resolution fluorescence imaging of cellular structures. Expansion (EXM) is a method physical expansion to obtain super-resolution images biological sample on conventional microscopy. We present yeast organelles, applying the combination and ExM techniques. When preparing pre-expanded samples, methods lead breakage dividing cells difficulties studying division-related proteins. Here, we describe an improved preparation technique avoids such damage. with Airyscan structured illumination (SIM) collected sub-cellular structural nuclear pore complex, septin, a-tubulin yeast. Our well-suited for study